Plasmid

Part:BBa_K3505010:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-20)


pDGB1 omega 1R (Ω1R) for Bacterial GoldenBraid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3097
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 6
    Illegal PstI site found at 3348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3097
    Illegal EcoRI site found at 3387
    Illegal PstI site found at 6
    Illegal PstI site found at 3348
    Illegal NotI site found at 3103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3097
    Illegal EcoRI site found at 3387
    Illegal BamHI site found at 3127
    Illegal BamHI site found at 3366
    Illegal BamHI site found at 3748
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3097
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 6
    Illegal PstI site found at 3348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3097
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 6
    Illegal PstI site found at 3348
    Illegal NgoMIV site found at 1019
    Illegal NgoMIV site found at 1421
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3133
    Illegal BsaI.rc site found at 3742